MIES is a proven [1, 2, 3, 4, 5, 6] sweep based data acquisition software package for intracellular electrophysiology (patch clamp). It offers top of its class flexibility and robustness for stimulus generation, data acquisition, and analysis.

Highlights

• Acquire data on up to eight headstage on a single DAC.
• Run up to five DACs in parallel.
• Create arbitrarily complex stimulus sets with an easy to use GUI
• Export all data, including all of its metadata, into the industry-standard NWBv2-format and read it back in
• Run custom code during data acquisition for Automatic Experiment Control
• Completely automate the experimental setup using JSON configuration files
• Interact with MIES from other programming languages (Python, C++, Javascript, ...) using ZeroMQ.
• Comprehensive experimental metadata acquisition and browsing tools
• Use the integrated scripting language for convenient on-the-fly data evaluation.
• Fully backward compatible with every earlier MIES version

Supported Hardware

• Digital to analog converters (DAC):

  • National Instruments:

    • PCIe-6341
    • PCIe-6343
    • PXI-6259
    • USB-6346

    Other NI hardware models can be added on request. Please open an issue from within MIES, MIES Panels->Report an issue, for that.

  • Instrutech/HEKA ITC:

    • 16
    • 18
    • 1600

    ITC devices are at the End-Of-Service-Life (EOSL). National Instruments DACs are recommended for MIES users looking for new hardware.

• Amplifier: Molecular Devices 700B

• Pressure control (optional): ITC 18/1600 or National Instruments USB 6001

Required Software

• Igor Pro 9 (nightly) or later

Acquisition

• Windows 10 x64
• NIDAQ MX XOP

Data evaluation

• Windows 10x64 or MacOSX 10.10

Getting started

• Download the latest release
• Windows: Run the installer
• MacOSX (Analysis only): Manual installation
• View the documentation

Support statement

The last released version receives fixes for all critical bugs.

Bug reporting

Instructions