diff --git a/packages/coverage-istanbul/src/index.ts b/packages/coverage-istanbul/src/index.ts
index a88aae227082..de791bc69b33 100644
--- a/packages/coverage-istanbul/src/index.ts
+++ b/packages/coverage-istanbul/src/index.ts
@@ -7,5 +7,11 @@ export async function getProvider() {
 
 export function takeCoverage() {
   // @ts-expect-error -- untyped global
-  return globalThis[COVERAGE_STORE_KEY]
+  const coverage = globalThis[COVERAGE_STORE_KEY]
+
+  // Reset coverage map to prevent duplicate results if this is called twice in row
+  // @ts-expect-error -- untyped global
+  globalThis[COVERAGE_STORE_KEY] = {}
+
+  return coverage
 }
diff --git a/test/coverage-test/coverage-test/coverage.istanbul.test.ts b/test/coverage-test/coverage-test/coverage.istanbul.test.ts
index ead65978cbf4..6b3858994fde 100644
--- a/test/coverage-test/coverage-test/coverage.istanbul.test.ts
+++ b/test/coverage-test/coverage-test/coverage.istanbul.test.ts
@@ -40,8 +40,8 @@ test('istanbul json report', async () => {
   const normalizedReport: CoverageFinalJson['default'] = {}
 
   for (const [filename, coverage] of Object.entries(jsonReport)) {
-    coverage.path = normalize(coverage.path.replace(process.cwd(), '<root>'))
-    normalizedReport[filename.replace(process.cwd(), '<root>')] = coverage
+    coverage.path = normalizeFilename(coverage.path)
+    normalizedReport[normalizeFilename(filename)] = coverage
   }
 
   expect(normalizedReport).toMatchSnapshot()
@@ -81,3 +81,7 @@ test('file using import.meta.env is included in report', async () => {
 
   expect(files).toContain('importEnv.ts.html')
 })
+
+function normalizeFilename(filename: string) {
+  return normalize(filename.replace(process.cwd(), '<root>'))
+}