diff --git a/CHANGELOG.md b/CHANGELOG.md
index 5722c256a4..b144d46e19 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -17,6 +17,7 @@ We use *breaking :warning:* to mark changes that are not backward compatible (re
 ### Added
 
 - [#5337](https://github.com/thanos-io/thanos/pull/5337) Thanos Object Store: Add the `prefix` option to buckets
+- [#5409](https://github.com/thanos-io/thanos/pull/5409) S3: Add option to force DNS style lookup.
 - [#5352](https://github.com/thanos-io/thanos/pull/5352) Cache: Add cache metrics to groupcache.
 - [#5391](https://github.com/thanos-io/thanos/pull/5391) Receive: Add relabeling support.
 - [#5408](https://github.com/thanos-io/thanos/pull/5391) Receive: Add support for consistent hashrings.
diff --git a/docs/storage.md b/docs/storage.md
index 47f2bd3f19..b2f0316537 100644
--- a/docs/storage.md
+++ b/docs/storage.md
@@ -89,6 +89,7 @@ config:
   trace:
     enable: false
   list_objects_version: ""
+  bucket_lookup_type: auto
   part_size: 67108864
   sse_config:
     type: ""
@@ -119,6 +120,8 @@ Set `list_objects_version: "v1"` for S3 compatible APIs that don't support ListO
 
 `http_config.tls_config` allows configuring TLS connections. Please refer to the document of [tls_config](https://prometheus.io/docs/prometheus/latest/configuration/configuration/#tls_config) for detailed information on what each option does.
 
+`bucket_lookup_type` can be `auto`, `virtual-hosted` or `path`. Read more about it [here](https://docs.aws.amazon.com/AmazonS3/latest/userguide/VirtualHosting.html).
+
 For debug and testing purposes you can set
 
 * `insecure: true` to switch to plain insecure HTTP instead of HTTPS
diff --git a/pkg/objstore/s3/s3.go b/pkg/objstore/s3/s3.go
index 1ece1a51f8..1e0ae3fe62 100644
--- a/pkg/objstore/s3/s3.go
+++ b/pkg/objstore/s3/s3.go
@@ -34,7 +34,50 @@ import (
 
 type ctxKey int
 
+type BucketLookupType int
+
+func (blt BucketLookupType) String() string {
+	return []string{"auto", "virtual-hosted", "path"}[blt]
+}
+
+func (blt BucketLookupType) MinioType() minio.BucketLookupType {
+	return []minio.BucketLookupType{
+		minio.BucketLookupAuto,
+		minio.BucketLookupDNS,
+		minio.BucketLookupPath,
+	}[blt]
+}
+
+func (blt BucketLookupType) MarshalYAML() (interface{}, error) {
+	return blt.String(), nil
+}
+
+func (blt *BucketLookupType) UnmarshalYAML(unmarshal func(interface{}) error) error {
+	var lookupType string
+	if err := unmarshal(&lookupType); err != nil {
+		return err
+	}
+
+	switch lookupType {
+	case "auto":
+		*blt = AutoLookup
+		return nil
+	case "virtual-hosted":
+		*blt = VirtualHostLookup
+		return nil
+	case "path":
+		*blt = PathLookup
+		return nil
+	}
+
+	return fmt.Errorf("unsupported bucket lookup type: %s", lookupType)
+}
+
 const (
+	AutoLookup BucketLookupType = iota
+	VirtualHostLookup
+	PathLookup
+
 	// DirDelim is the delimiter used to model a directory structure in an object store bucket.
 	DirDelim = "/"
 
@@ -66,7 +109,8 @@ var DefaultConfig = Config{
 		MaxIdleConnsPerHost:   100,
 		MaxConnsPerHost:       0,
 	},
-	PartSize: 1024 * 1024 * 64, // 64MB.
+	PartSize:         1024 * 1024 * 64, // 64MB.
+	BucketLookupType: AutoLookup,
 }
 
 // Config stores the configuration for s3 bucket.
@@ -83,6 +127,7 @@ type Config struct {
 	HTTPConfig         HTTPConfig        `yaml:"http_config"`
 	TraceConfig        TraceConfig       `yaml:"trace"`
 	ListObjectsVersion string            `yaml:"list_objects_version"`
+	BucketLookupType   BucketLookupType  `yaml:"bucket_lookup_type"`
 	// PartSize used for multipart upload. Only used if uploaded object size is known and larger than configured PartSize.
 	// NOTE we need to make sure this number does not produce more parts than 10 000.
 	PartSize    uint64    `yaml:"part_size"`
@@ -265,10 +310,11 @@ func NewBucketWithConfig(logger log.Logger, config Config, component string) (*B
 	}
 
 	client, err := minio.New(config.Endpoint, &minio.Options{
-		Creds:     credentials.NewChainCredentials(chain),
-		Secure:    !config.Insecure,
-		Region:    config.Region,
-		Transport: rt,
+		Creds:        credentials.NewChainCredentials(chain),
+		Secure:       !config.Insecure,
+		Region:       config.Region,
+		Transport:    rt,
+		BucketLookup: config.BucketLookupType.MinioType(),
 	})
 	if err != nil {
 		return nil, errors.Wrap(err, "initialize s3 client")
diff --git a/test/e2e/e2ethanos/services.go b/test/e2e/e2ethanos/services.go
index 587d9e05c3..a2d62f8f09 100644
--- a/test/e2e/e2ethanos/services.go
+++ b/test/e2e/e2ethanos/services.go
@@ -959,6 +959,7 @@ func NewS3Config(bucket, endpoint, basePath string) s3.Config {
 				KeyFile:  filepath.Join(basePath, "certs", "private.key"),
 			},
 		},
+		BucketLookupType: s3.AutoLookup,
 	}
 }