diff --git a/src/OptionList.tsx b/src/OptionList.tsx
index e151941f0..d3864c56d 100644
--- a/src/OptionList.tsx
+++ b/src/OptionList.tsx
@@ -10,6 +10,7 @@ import {
   FlattenOptionData as SelectFlattenOptionData,
   OptionData,
   RenderNode,
+  OnActiveValue,
 } from './interface';
 import { RawValueType, FlattenOptionsType } from './interface/generator';
 
@@ -33,7 +34,7 @@ export interface OptionListProps<OptionsType extends object[]> {
   onSelect: (value: RawValueType, option: { selected: boolean }) => void;
   onToggleOpen: (open?: boolean) => void;
   /** Tell Select that some value is now active to make accessibility work */
-  onActiveValue: (value: RawValueType, index: number) => void;
+  onActiveValue: OnActiveValue;
   onScroll: React.UIEventHandler<HTMLDivElement>;
 
   /** Tell Select that mouse enter the popup to force re-render */
@@ -115,17 +116,19 @@ const OptionList: React.RefForwardingComponent<
   };
 
   const [activeIndex, setActiveIndex] = React.useState(() => getEnabledActiveIndex(0));
-  const setActive = (index: number) => {
+  const setActive = (index: number, fromKeyboard = false) => {
     setActiveIndex(index);
 
+    const info = { source: fromKeyboard ? ('keyboard' as const) : ('mouse' as const) };
+
     // Trigger active event
     const flattenItem = memoFlattenOptions[index];
     if (!flattenItem) {
-      onActiveValue(null, -1);
+      onActiveValue(null, -1, info);
       return;
     }
 
-    onActiveValue((flattenItem.data as OptionData).value, index);
+    onActiveValue((flattenItem.data as OptionData).value, index, info);
   };
 
   // Auto active first item when list length or searchValue changed
@@ -184,7 +187,7 @@ const OptionList: React.RefForwardingComponent<
           if (offset !== 0) {
             const nextActiveIndex = getEnabledActiveIndex(activeIndex + offset, offset);
             scrollIntoView(nextActiveIndex);
-            setActive(nextActiveIndex);
+            setActive(nextActiveIndex, true);
           }
 
           break;
diff --git a/src/generate.tsx b/src/generate.tsx
index 4dabeb8a5..5b053a630 100644
--- a/src/generate.tsx
+++ b/src/generate.tsx
@@ -14,7 +14,7 @@ import classNames from 'classnames';
 import useMergedState from 'rc-util/lib/hooks/useMergedState';
 import Selector, { RefSelectorProps } from './Selector';
 import SelectTrigger, { RefTriggerProps } from './SelectTrigger';
-import { RenderNode, Mode, RenderDOMFunc } from './interface';
+import { RenderNode, Mode, RenderDOMFunc, OnActiveValue } from './interface';
 import {
   GetLabeledValue,
   FilterOptions,
@@ -187,11 +187,10 @@ export interface GenerateConfig<OptionsType extends object[]> {
   /** Convert single raw value into { label, value } format. Will be called by each value */
   getLabeledValue: GetLabeledValue<FlattenOptionsType<OptionsType>>;
   filterOptions: FilterOptions<OptionsType>;
-  findValueOption:
-    | (// Need still support legacy ts api
-      (values: RawValueType[], options: FlattenOptionsType<OptionsType>) => OptionsType)
-    | (// New API add prevValueOptions support
-      (
+  findValueOption: // Need still support legacy ts api
+    | ((values: RawValueType[], options: FlattenOptionsType<OptionsType>) => OptionsType)
+    // New API add prevValueOptions support
+    | ((
         values: RawValueType[],
         options: FlattenOptionsType<OptionsType>,
         info?: { prevValueOptions?: OptionsType[] },
@@ -874,10 +873,10 @@ export default function generateSelector<
     const mergedDefaultActiveFirstOption =
       defaultActiveFirstOption !== undefined ? defaultActiveFirstOption : mode !== 'combobox';
 
-    const onActiveValue = (active: RawValueType, index: number) => {
+    const onActiveValue: OnActiveValue = (active, index, { source = 'keyboard' } = {}) => {
       setAccessibilityIndex(index);
 
-      if (backfill && mode === 'combobox' && active !== null) {
+      if (backfill && mode === 'combobox' && active !== null && source === 'keyboard') {
         setActiveValue(String(active));
       }
     };
diff --git a/src/interface/index.ts b/src/interface/index.ts
index f259f13a2..42456cae4 100644
--- a/src/interface/index.ts
+++ b/src/interface/index.ts
@@ -1,5 +1,5 @@
 import * as React from 'react';
-import { Key } from './generator';
+import { Key, RawValueType } from './generator';
 
 export type RenderDOMFunc = (props: any) => HTMLElement;
 
@@ -8,6 +8,12 @@ export type RenderNode = React.ReactNode | ((props: any) => React.ReactNode);
 export type Mode = 'multiple' | 'tags' | 'combobox';
 
 // ======================== Option ========================
+export type OnActiveValue = (
+  active: RawValueType,
+  index: number,
+  info?: { source?: 'keyboard' | 'mouse' },
+) => void;
+
 export interface OptionCoreData {
   key?: Key;
   disabled?: boolean;
diff --git a/tests/Combobox.test.tsx b/tests/Combobox.test.tsx
index ad4fba4e7..44ddc4cee 100644
--- a/tests/Combobox.test.tsx
+++ b/tests/Combobox.test.tsx
@@ -235,6 +235,22 @@ describe('Select.Combobox', () => {
       expect(handleSelect).toHaveBeenCalledWith('One', expect.objectContaining({ value: 'One' }));
     });
 
+    it('mouse should not trigger', () => {
+      const wrapper = mount(
+        <Select mode="combobox" backfill open>
+          <Option value="One">One</Option>
+          <Option value="Two">Two</Option>
+        </Select>,
+      );
+
+      wrapper
+        .find('.rc-select-item-option')
+        .first()
+        .simulate('mouseMove');
+
+      expect(wrapper.find('input').props().value).toBeFalsy();
+    });
+
     // https://github.com/ant-design/ant-design/issues/25345
     it('dynamic options', () => {
       const onChange = jest.fn();
diff --git a/tests/OptionList.test.tsx b/tests/OptionList.test.tsx
index fef0a65d3..350ba02f3 100644
--- a/tests/OptionList.test.tsx
+++ b/tests/OptionList.test.tsx
@@ -68,7 +68,7 @@ describe('OptionList', () => {
       }),
     );
 
-    expect(onActiveValue).toHaveBeenCalledWith('1', expect.anything());
+    expect(onActiveValue).toHaveBeenCalledWith('1', expect.anything(), expect.anything());
   });
 
   it('key operation', () => {
@@ -86,13 +86,21 @@ describe('OptionList', () => {
     act(() => {
       listRef.current.onKeyDown({ which: KeyCode.DOWN } as any);
     });
-    expect(onActiveValue).toHaveBeenCalledWith('2', expect.anything());
+    expect(onActiveValue).toHaveBeenCalledWith(
+      '2',
+      expect.anything(),
+      expect.objectContaining({ source: 'keyboard' }),
+    );
 
     onActiveValue.mockReset();
     act(() => {
       listRef.current.onKeyDown({ which: KeyCode.UP } as any);
     });
-    expect(onActiveValue).toHaveBeenCalledWith('1', expect.anything());
+    expect(onActiveValue).toHaveBeenCalledWith(
+      '1',
+      expect.anything(),
+      expect.objectContaining({ source: 'keyboard' }),
+    );
   });
 
   it('hover to active', () => {
@@ -109,7 +117,11 @@ describe('OptionList', () => {
       .find('.rc-select-item-option')
       .last()
       .simulate('mouseMove');
-    expect(onActiveValue).toHaveBeenCalledWith('2', expect.anything());
+    expect(onActiveValue).toHaveBeenCalledWith(
+      '2',
+      expect.anything(),
+      expect.objectContaining({ source: 'mouse' }),
+    );
 
     // Same item not repeat trigger
     onActiveValue.mockReset();