diff --git a/Tests/images/tiff_16bit_RGB.tiff b/Tests/images/tiff_16bit_RGB.tiff
new file mode 100644
index 00000000000..5eb7c73c245
Binary files /dev/null and b/Tests/images/tiff_16bit_RGB.tiff differ
diff --git a/Tests/images/tiff_16bit_RGB_target.png b/Tests/images/tiff_16bit_RGB_target.png
new file mode 100644
index 00000000000..9235800043b
Binary files /dev/null and b/Tests/images/tiff_16bit_RGB_target.png differ
diff --git a/Tests/test_file_libtiff.py b/Tests/test_file_libtiff.py
index 1d30393d4df..4bcf1e116c9 100644
--- a/Tests/test_file_libtiff.py
+++ b/Tests/test_file_libtiff.py
@@ -631,6 +631,20 @@ def test_save_tiff_with_jpegtables(self):
         # Should not raise UnicodeDecodeError or anything else
         im.save(outfile)
 
+    def test_16bit_RGB_tiff(self):
+        im = Image.open("Tests/images/tiff_16bit_RGB.tiff")
+
+        self.assertEqual(im.mode, "RGB")
+        self.assertEqual(im.size, (100, 40))
+        self.assertEqual(
+            im.tile,
+            [('tiff_adobe_deflate', (0, 0, 100, 40), 0, ('RGB;16N', 'tiff_adobe_deflate', False))]
+        )
+        im.load()
+
+        self.assert_image_equal_tofile(
+            im, "Tests/images/tiff_16bit_RGB_target.png")
+
     def test_16bit_RGBa_tiff(self):
         im = Image.open("Tests/images/tiff_16bit_RGBa.tiff")
 
diff --git a/src/libImaging/Unpack.c b/src/libImaging/Unpack.c
index d9a948b3246..02196d25559 100644
--- a/src/libImaging/Unpack.c
+++ b/src/libImaging/Unpack.c
@@ -1369,12 +1369,12 @@ static struct {
     {"RGBA",    "A",            8,      band3},
 
 #ifdef WORDS_BIGENDIAN
-    {"RGB",     "RGB;16N",      64,     unpackRGB16B},
+    {"RGB",     "RGB;16N",      48,     unpackRGB16B},
     {"RGBA",    "RGBa;16N",     64,     unpackRGBa16B},
     {"RGBA",    "RGBA;16N",     64,     unpackRGBA16B},
     {"RGBX",    "RGBX;16N",     64,     unpackRGBA16B},
 #else
-    {"RGB",     "RGB;16N",      64,     unpackRGB16L},
+    {"RGB",     "RGB;16N",      48,     unpackRGB16L},
     {"RGBA",    "RGBa;16N",     64,     unpackRGBa16L},
     {"RGBA",    "RGBA;16N",     64,     unpackRGBA16L},
     {"RGBX",    "RGBX;16N",     64,     unpackRGBA16B},