diff --git a/Tests/images/duplicate_number_of_loops.gif b/Tests/images/duplicate_number_of_loops.gif
new file mode 100644
index 00000000000..ac315ee99f3
Binary files /dev/null and b/Tests/images/duplicate_number_of_loops.gif differ
diff --git a/Tests/test_file_gif.py b/Tests/test_file_gif.py
index dffd1006f2f..be28364d295 100644
--- a/Tests/test_file_gif.py
+++ b/Tests/test_file_gif.py
@@ -742,9 +742,16 @@ def test_number_of_loops(tmp_path):
     im = Image.new("L", (100, 100), "#000")
     im.save(out, loop=number_of_loops)
     with Image.open(out) as reread:
-
         assert reread.info["loop"] == number_of_loops
 
+    # Check that even if a subsequent GIF frame has the number of loops specified,
+    # only the value from the first frame is used
+    with Image.open("Tests/images/duplicate_number_of_loops.gif") as im:
+        assert im.info["loop"] == 2
+
+        im.seek(1)
+        assert im.info["loop"] == 2
+
 
 def test_background(tmp_path):
     out = str(tmp_path / "temp.gif")
diff --git a/src/PIL/GifImagePlugin.py b/src/PIL/GifImagePlugin.py
index 2c885375523..d5c0f74e24c 100644
--- a/src/PIL/GifImagePlugin.py
+++ b/src/PIL/GifImagePlugin.py
@@ -236,7 +236,7 @@ def _seek(self, frame, update_image=True):
                         block = self.data()
                     s = None
                     continue
-                elif s[0] == 255:
+                elif s[0] == 255 and frame == 0:
                     #
                     # application extension
                     #
@@ -244,7 +244,7 @@ def _seek(self, frame, update_image=True):
                     if block[:11] == b"NETSCAPE2.0":
                         block = self.data()
                         if len(block) >= 3 and block[0] == 1:
-                            info["loop"] = i16(block, 1)
+                            self.info["loop"] = i16(block, 1)
                 while self.data():
                     pass
 
@@ -389,7 +389,7 @@ def _rgb(color):
                 )
             ]
 
-        for k in ["duration", "comment", "extension", "loop"]:
+        for k in ["duration", "comment", "extension"]:
             if k in info:
                 self.info[k] = info[k]
             elif k in self.info: