diff --git a/src/_pytest/compat.py b/src/_pytest/compat.py
index 52051ff2326..c3ecaf9121d 100644
--- a/src/_pytest/compat.py
+++ b/src/_pytest/compat.py
@@ -228,12 +228,31 @@ def ascii_escaped(val):
             return val.encode("unicode-escape")
 
 
+class _PytestWrapper(object):
+    """Dummy wrapper around a function object for internal use only.
+
+    Used to correctly unwrap the underlying function object
+    when we are creating fixtures, because we wrap the function object ourselves with a decorator
+    to issue warnings when the fixture function is called directly.
+    """
+
+    def __init__(self, obj):
+        self.obj = obj
+
+
 def get_real_func(obj):
     """ gets the real function object of the (possibly) wrapped object by
     functools.wraps or functools.partial.
     """
     start_obj = obj
     for i in range(100):
+        # __pytest_wrapped__ is set by @pytest.fixture when wrapping the fixture function
+        # to trigger a warning if it gets called directly instead of by pytest: we don't
+        # want to unwrap further than this otherwise we lose useful wrappings like @mock.patch (#3774)
+        new_obj = getattr(obj, "__pytest_wrapped__", None)
+        if isinstance(new_obj, _PytestWrapper):
+            obj = new_obj.obj
+            break
         new_obj = getattr(obj, "__wrapped__", None)
         if new_obj is None:
             break
diff --git a/src/_pytest/fixtures.py b/src/_pytest/fixtures.py
index 818c5b81f74..a6634cd1162 100644
--- a/src/_pytest/fixtures.py
+++ b/src/_pytest/fixtures.py
@@ -31,6 +31,7 @@
     safe_getattr,
     FuncargnamesCompatAttr,
     get_real_method,
+    _PytestWrapper,
 )
 from _pytest.deprecated import FIXTURE_FUNCTION_CALL, RemovedInPytest4Warning
 from _pytest.outcomes import fail, TEST_OUTCOME
@@ -954,9 +955,6 @@ def _ensure_immutable_ids(ids):
 def wrap_function_to_warning_if_called_directly(function, fixture_marker):
     """Wrap the given fixture function so we can issue warnings about it being called directly, instead of
     used as an argument in a test function.
-
-    The warning is emitted only in Python 3, because I didn't find a reliable way to make the wrapper function
-    keep the original signature, and we probably will drop Python 2 in Pytest 4 anyway.
     """
     is_yield_function = is_generator(function)
     msg = FIXTURE_FUNCTION_CALL.format(name=fixture_marker.name or function.__name__)
@@ -982,6 +980,10 @@ def result(*args, **kwargs):
     if six.PY2:
         result.__wrapped__ = function
 
+    # keep reference to the original function in our own custom attribute so we don't unwrap
+    # further than this point and lose useful wrappings like @mock.patch (#3774)
+    result.__pytest_wrapped__ = _PytestWrapper(function)
+
     return result
 
 
diff --git a/testing/acceptance_test.py b/testing/acceptance_test.py
index 6f12cc3351a..bc4e3bed85f 100644
--- a/testing/acceptance_test.py
+++ b/testing/acceptance_test.py
@@ -1044,3 +1044,10 @@ def test2():
     )
     result = testdir.runpytest_subprocess()
     result.stdout.fnmatch_lines(["*1 failed, 1 passed in*"])
+
+
+def test_fixture_mock_integration(testdir):
+    """Test that decorators applied to fixture are left working (#3774)"""
+    p = testdir.copy_example("acceptance/fixture_mock_integration.py")
+    result = testdir.runpytest(p)
+    result.stdout.fnmatch_lines("*1 passed*")
diff --git a/testing/example_scripts/acceptance/fixture_mock_integration.py b/testing/example_scripts/acceptance/fixture_mock_integration.py
new file mode 100644
index 00000000000..51f46f82cae
--- /dev/null
+++ b/testing/example_scripts/acceptance/fixture_mock_integration.py
@@ -0,0 +1,17 @@
+"""Reproduces issue #3774"""
+
+import mock
+
+import pytest
+
+config = {"mykey": "ORIGINAL"}
+
+
+@pytest.fixture(scope="function")
+@mock.patch.dict(config, {"mykey": "MOCKED"})
+def my_fixture():
+    return config["mykey"]
+
+
+def test_foobar(my_fixture):
+    assert my_fixture == "MOCKED"
diff --git a/testing/test_compat.py b/testing/test_compat.py
index 399b0d342b5..a6249d14b2e 100644
--- a/testing/test_compat.py
+++ b/testing/test_compat.py
@@ -1,8 +1,11 @@
 from __future__ import absolute_import, division, print_function
 import sys
+from functools import wraps
+
+import six
 
 import pytest
-from _pytest.compat import is_generator, get_real_func, safe_getattr
+from _pytest.compat import is_generator, get_real_func, safe_getattr, _PytestWrapper
 from _pytest.outcomes import OutcomeException
 
 
@@ -38,6 +41,33 @@ def __getattr__(self, attr):
         print(res)
 
 
+def test_get_real_func():
+    """Check that get_real_func correctly unwraps decorators until reaching the real function"""
+
+    def decorator(f):
+        @wraps(f)
+        def inner():
+            pass
+
+        if six.PY2:
+            inner.__wrapped__ = f
+        return inner
+
+    def func():
+        pass
+
+    wrapped_func = decorator(decorator(func))
+    assert get_real_func(wrapped_func) is func
+
+    wrapped_func2 = decorator(decorator(wrapped_func))
+    assert get_real_func(wrapped_func2) is func
+
+    # special case for __pytest_wrapped__ attribute: used to obtain the function up until the point
+    # a function was wrapped by pytest itself
+    wrapped_func2.__pytest_wrapped__ = _PytestWrapper(wrapped_func)
+    assert get_real_func(wrapped_func2) is wrapped_func
+
+
 @pytest.mark.skipif(
     sys.version_info < (3, 4), reason="asyncio available in Python 3.4+"
 )