diff --git a/src/_pytest/pytester.py b/src/_pytest/pytester.py
index 94058f70cba..43018f38f2c 100644
--- a/src/_pytest/pytester.py
+++ b/src/_pytest/pytester.py
@@ -1319,8 +1319,7 @@ def fnmatch_lines(self, lines2):
 
         The argument is a list of lines which have to match and can use glob
         wildcards.  If they do not match a pytest.fail() is called.  The
-        matches and non-matches are also printed on stdout.
-
+        matches and non-matches are also shown as part of the error message.
         """
         __tracebackhide__ = True
         self._match_lines(lines2, fnmatch, "fnmatch")
@@ -1331,8 +1330,7 @@ def re_match_lines(self, lines2):
         The argument is a list of lines which have to match using ``re.match``.
         If they do not match a pytest.fail() is called.
 
-        The matches and non-matches are also printed on stdout.
-
+        The matches and non-matches are also shown as part of the error message.
         """
         __tracebackhide__ = True
         self._match_lines(lines2, lambda name, pat: re.match(pat, name), "re.match")
@@ -1375,3 +1373,40 @@ def _match_lines(self, lines2, match_func, match_nickname):
             else:
                 self._log("remains unmatched: {!r}".format(line))
                 pytest.fail(self._log_text)
+
+    def no_fnmatch_line(self, pat):
+        """Ensure captured lines do not match the given pattern, using ``fnmatch.fnmatch``.
+
+        :param str pat: the pattern to match lines.
+        """
+        __tracebackhide__ = True
+        self._no_match_line(pat, fnmatch, "fnmatch")
+
+    def no_re_match_line(self, pat):
+        """Ensure captured lines do not match the given pattern, using ``re.match``.
+
+        :param str pat: the regular expression to match lines.
+        """
+        __tracebackhide__ = True
+        self._no_match_line(pat, lambda name, pat: re.match(pat, name), "re.match")
+
+    def _no_match_line(self, pat, match_func, match_nickname):
+        """Ensure captured lines does not have a the given pattern, using ``fnmatch.fnmatch``
+
+        :param str pat: the pattern to match lines
+        """
+        __tracebackhide__ = True
+        nomatch_printed = False
+        try:
+            for line in self.lines:
+                if match_func(line, pat):
+                    self._log("%s:" % match_nickname, repr(pat))
+                    self._log("   with:", repr(line))
+                    pytest.fail(self._log_text)
+                else:
+                    if not nomatch_printed:
+                        self._log("nomatch:", repr(pat))
+                        nomatch_printed = True
+                    self._log("    and:", repr(line))
+        finally:
+            self._log_output = []
diff --git a/testing/test_pytester.py b/testing/test_pytester.py
index d330ff2532b..f8b0896c5fd 100644
--- a/testing/test_pytester.py
+++ b/testing/test_pytester.py
@@ -457,6 +457,52 @@ def test_linematcher_with_nonlist():
     assert lm._getlines(set()) == set()
 
 
+@pytest.mark.parametrize("function", ["no_fnmatch_line", "no_re_match_line"])
+def test_no_matching(function):
+    """"""
+    if function == "no_fnmatch_line":
+        match_func_name = "fnmatch"
+        good_pattern = "*.py OK*"
+        bad_pattern = "*X.py OK*"
+    else:
+        assert function == "no_re_match_line"
+        match_func_name = "re.match"
+        good_pattern = r".*py OK"
+        bad_pattern = r".*Xpy OK"
+
+    lm = LineMatcher(
+        [
+            "cachedir: .pytest_cache",
+            "collecting ... collected 1 item",
+            "",
+            "show_fixtures_per_test.py OK",
+            "=== elapsed 1s ===",
+        ]
+    )
+
+    def check_failure_lines(lines):
+        expected = [
+            "nomatch: '{}'".format(good_pattern),
+            "    and: 'cachedir: .pytest_cache'",
+            "    and: 'collecting ... collected 1 item'",
+            "    and: ''",
+            "{}: '{}'".format(match_func_name, good_pattern),
+            "   with: 'show_fixtures_per_test.py OK'",
+        ]
+        assert lines == expected
+
+    # check the function twice to ensure we don't accumulate the internal buffer
+    for i in range(2):
+        with pytest.raises(pytest.fail.Exception) as e:
+            func = getattr(lm, function)
+            func(good_pattern)
+        obtained = str(e.value).splitlines()
+        check_failure_lines(obtained)
+
+    func = getattr(lm, function)
+    func(bad_pattern)  # bad pattern does not match any line: passes
+
+
 def test_pytester_addopts(request, monkeypatch):
     monkeypatch.setenv("PYTEST_ADDOPTS", "--orig-unused")