diff --git a/CHANGES.md b/CHANGES.md
index ba9f4c06f28..b1e8f6c75b1 100644
--- a/CHANGES.md
+++ b/CHANGES.md
@@ -18,6 +18,9 @@
 
 <!-- Changes to how Black can be configured -->
 
+- Fix incorrectly ignoring .gitignore presence when more than one source directory is
+  specified (#3336)
+
 ### Packaging
 
 <!-- Changes to how Black is packaged, such as dependency requirements -->
diff --git a/src/black/__init__.py b/src/black/__init__.py
index 5293796aea1..2e43e800c40 100644
--- a/src/black/__init__.py
+++ b/src/black/__init__.py
@@ -30,6 +30,7 @@
 import click
 from click.core import ParameterSource
 from mypy_extensions import mypyc_attr
+from pathspec import PathSpec
 from pathspec.patterns.gitwildmatch import GitWildMatchPatternError
 
 from _black_version import version as __version__
@@ -625,6 +626,11 @@ def get_sources(
     sources: Set[Path] = set()
     root = ctx.obj["root"]
 
+    exclude_is_None = exclude is None
+    exclude = re_compile_maybe_verbose(DEFAULT_EXCLUDES) if exclude is None else exclude
+    gitignore = None  # type: Optional[PathSpec]
+    root_gitignore = get_gitignore(root)
+
     for s in src:
         if s == "-" and stdin_filename:
             p = Path(stdin_filename)
@@ -658,16 +664,14 @@ def get_sources(
 
             sources.add(p)
         elif p.is_dir():
-            if exclude is None:
-                exclude = re_compile_maybe_verbose(DEFAULT_EXCLUDES)
-                gitignore = get_gitignore(root)
+            if exclude_is_None:
                 p_gitignore = get_gitignore(p)
                 # No need to use p's gitignore if it is identical to root's gitignore
                 # (i.e. root and p point to the same directory).
-                if gitignore != p_gitignore:
-                    gitignore += p_gitignore
-            else:
-                gitignore = None
+                if root_gitignore == p_gitignore:
+                    gitignore = root_gitignore
+                else:
+                    gitignore = root_gitignore + p_gitignore
             sources.update(
                 gen_python_files(
                     p.iterdir(),
diff --git a/tests/data/gitignore_used_on_multiple_sources/.gitignore b/tests/data/gitignore_used_on_multiple_sources/.gitignore
new file mode 100644
index 00000000000..2987e7bb646
--- /dev/null
+++ b/tests/data/gitignore_used_on_multiple_sources/.gitignore
@@ -0,0 +1 @@
+a.py
diff --git a/tests/data/gitignore_used_on_multiple_sources/dir1/a.py b/tests/data/gitignore_used_on_multiple_sources/dir1/a.py
new file mode 100644
index 00000000000..e69de29bb2d
diff --git a/tests/data/gitignore_used_on_multiple_sources/dir1/b.py b/tests/data/gitignore_used_on_multiple_sources/dir1/b.py
new file mode 100644
index 00000000000..e69de29bb2d
diff --git a/tests/data/gitignore_used_on_multiple_sources/dir2/a.py b/tests/data/gitignore_used_on_multiple_sources/dir2/a.py
new file mode 100644
index 00000000000..e69de29bb2d
diff --git a/tests/data/gitignore_used_on_multiple_sources/dir2/b.py b/tests/data/gitignore_used_on_multiple_sources/dir2/b.py
new file mode 100644
index 00000000000..e69de29bb2d
diff --git a/tests/test_black.py b/tests/test_black.py
index 5d0175d9d66..d66305becf0 100644
--- a/tests/test_black.py
+++ b/tests/test_black.py
@@ -1956,6 +1956,17 @@ def test_gitignore_used_as_default(self) -> None:
         ctx.obj["root"] = base
         assert_collected_sources(src, expected, ctx=ctx, extend_exclude=r"/exclude/")
 
+    def test_gitignore_used_on_multiple_sources(self) -> None:
+        root = Path(DATA_DIR / "gitignore_used_on_multiple_sources")
+        expected = [
+            root / "dir1" / "b.py",
+            root / "dir2" / "b.py",
+        ]
+        ctx = FakeContext()
+        ctx.obj["root"] = root
+        src = [root / "dir1", root / "dir2"]
+        assert_collected_sources(src, expected, ctx=ctx)
+
     @patch("black.find_project_root", lambda *args: (THIS_DIR.resolve(), None))
     def test_exclude_for_issue_1572(self) -> None:
         # Exclude shouldn't touch files that were explicitly given to Black through the