forked from biopython/biopython
/
pairwise2_testCases.py
executable file
·911 lines (813 loc) · 26.5 KB
/
pairwise2_testCases.py
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
189
190
191
192
193
194
195
196
197
198
199
200
201
202
203
204
205
206
207
208
209
210
211
212
213
214
215
216
217
218
219
220
221
222
223
224
225
226
227
228
229
230
231
232
233
234
235
236
237
238
239
240
241
242
243
244
245
246
247
248
249
250
251
252
253
254
255
256
257
258
259
260
261
262
263
264
265
266
267
268
269
270
271
272
273
274
275
276
277
278
279
280
281
282
283
284
285
286
287
288
289
290
291
292
293
294
295
296
297
298
299
300
301
302
303
304
305
306
307
308
309
310
311
312
313
314
315
316
317
318
319
320
321
322
323
324
325
326
327
328
329
330
331
332
333
334
335
336
337
338
339
340
341
342
343
344
345
346
347
348
349
350
351
352
353
354
355
356
357
358
359
360
361
362
363
364
365
366
367
368
369
370
371
372
373
374
375
376
377
378
379
380
381
382
383
384
385
386
387
388
389
390
391
392
393
394
395
396
397
398
399
400
401
402
403
404
405
406
407
408
409
410
411
412
413
414
415
416
417
418
419
420
421
422
423
424
425
426
427
428
429
430
431
432
433
434
435
436
437
438
439
440
441
442
443
444
445
446
447
448
449
450
451
452
453
454
455
456
457
458
459
460
461
462
463
464
465
466
467
468
469
470
471
472
473
474
475
476
477
478
479
480
481
482
483
484
485
486
487
488
489
490
491
492
493
494
495
496
497
498
499
500
501
502
503
504
505
506
507
508
509
510
511
512
513
514
515
516
517
518
519
520
521
522
523
524
525
526
527
528
529
530
531
532
533
534
535
536
537
538
539
540
541
542
543
544
545
546
547
548
549
550
551
552
553
554
555
556
557
558
559
560
561
562
563
564
565
566
567
568
569
570
571
572
573
574
575
576
577
578
579
580
581
582
583
584
585
586
587
588
589
590
591
592
593
594
595
596
597
598
599
600
601
602
603
604
605
606
607
608
609
610
611
612
613
614
615
616
617
618
619
620
621
622
623
624
625
626
627
628
629
630
631
632
633
634
635
636
637
638
639
640
641
642
643
644
645
646
647
648
649
650
651
652
653
654
655
656
657
658
659
660
661
662
663
664
665
666
667
668
669
670
671
672
673
674
675
676
677
678
679
680
681
682
683
684
685
686
687
688
689
690
691
692
693
694
695
696
697
698
699
700
701
702
703
704
705
706
707
708
709
710
711
712
713
714
715
716
717
718
719
720
721
722
723
724
725
726
727
728
729
730
731
732
733
734
735
736
737
738
739
740
741
742
743
744
745
746
747
748
749
750
751
752
753
754
755
756
757
758
759
760
761
762
763
764
765
766
767
768
769
770
771
772
773
774
775
776
777
778
779
780
781
782
783
784
785
786
787
788
789
790
791
792
793
794
795
796
797
798
799
800
801
802
803
804
805
806
807
808
809
810
811
812
813
814
815
816
817
818
819
820
821
822
823
824
825
826
827
828
829
830
831
832
833
834
835
836
837
838
839
840
841
842
843
844
845
846
847
848
849
850
851
852
853
854
855
856
857
858
859
860
861
862
863
864
865
866
867
868
869
870
871
872
873
874
875
876
877
878
879
880
881
882
883
884
885
886
887
888
889
890
891
892
893
894
895
896
897
898
899
900
901
902
903
904
905
906
907
908
909
910
911
#!/usr/bin/env python
# This code is part of the Biopython distribution and governed by its
# license. Please see the LICENSE file that should have been included
# as part of this package.
"""Tests for pairwise2 module.
Put new test case here, the classes here will be imported and run
as TestCases in ``test_pairwise2.py`` and ``test_pairwise2_no_C.py``
with or without complementing C extensions.
"""
import unittest
import warnings
from Bio import BiopythonWarning
from Bio import pairwise2
from Bio.Align import substitution_matrices
class TestPairwiseErrorConditions(unittest.TestCase):
"""Test several error conditions."""
def test_function_name(self):
"""Test for wrong function names."""
# Function name pattern must be globalXX or localXX
self.assertRaises(AttributeError, lambda: pairwise2.align.globalxxx)
self.assertRaises(AttributeError, lambda: pairwise2.align.localxxx)
self.assertRaises(AttributeError, lambda: pairwise2.align.glocalxx)
# First X must be from (x, m, d, c), second from (x, s, d, c)
self.assertRaises(AttributeError, lambda: pairwise2.align.globalax)
self.assertRaises(AttributeError, lambda: pairwise2.align.globalxa)
def test_function_parameters(self):
"""Test for number of parameters."""
# globalxx takes two parameters
self.assertRaises(TypeError, pairwise2.align.globalxx, "A")
# matrix_only is no keyword argument
self.assertRaises(
TypeError, pairwise2.align.globalxx, "A", "C", {"matrix_only": True}
)
# Both sequences must be either strings or lists
self.assertRaises(TypeError, pairwise2.align.globalxx, "A", ["C"])
# If both sequences are lists, gap_char must also be set as list
self.assertRaises(TypeError, pairwise2.align.globalxx, ["A"], ["C"])
# If one or both sequences are empty, there is no alignment
alignment = pairwise2.align.globalxx("A", "")
self.assertEqual(alignment, [])
# Gap scores must be negativ
self.assertRaises(ValueError, pairwise2.align.globalxs, "A", "C", 5, -1)
self.assertRaises(ValueError, pairwise2.align.globalxs, "A", "C", -5, 1)
# Gap open penalty must be higher than gap extension penalty
self.assertRaises(ValueError, pairwise2.align.globalxs, "A", "C", -1, -5)
def test_param_names(self):
"""Test for unknown parameter in parameter names."""
a = pairwise2.align.alignment_function("globalxx")
a.param_names = ["Hello"]
self.assertRaises(ValueError, a.decode, "Bye")
def test_warnings(self):
"""Test for warnings."""
with warnings.catch_warnings(record=True) as w:
# Cause all warnings to always be triggered.
warnings.simplefilter("always")
# Trigger a warning.
pairwise2.align.localxx("GA", "CGA", penalize_end_gaps=True)
# Verify some things
self.assertEqual(len(w), 1)
self.assertEqual(w[-1].category, BiopythonWarning)
self.assertIn("should not", str(w[-1].message))
class TestPairwiseKeywordUsage(unittest.TestCase):
"""Tests for keyword usage."""
def test_keywords(self):
"""Test equality of calls with and without keywords."""
aligns = pairwise2.align.globalxx("GAACT", "GAT")
aligns_kw = pairwise2.align.globalxx(sequenceA="GAACT", sequenceB="GAT")
self.assertEqual(aligns, aligns_kw)
aligns = pairwise2.align.globalmx("GAACT", "GAT", 5, -4)
aligns_kw = pairwise2.align.globalmx(
sequenceA="GAACT", sequenceB="GAT", match=5, mismatch=-4
)
self.assertEqual(aligns, aligns_kw)
class TestPairwiseGlobal(unittest.TestCase):
"""Test some usual global alignments."""
def test_globalxx_simple(self):
"""Test globalxx."""
aligns = pairwise2.align.globalxx("GAACT", "GAT")
self.assertEqual(len(aligns), 2)
aligns.sort()
seq1, seq2, score, begin, end = aligns[0]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
GAACT
| | |
G-A-T
Score=3
""",
)
seq1, seq2, score, begin, end = aligns[1]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
GAACT
|| |
GA--T
Score=3
""",
)
def test_globalxx_simple2(self):
"""Do the same test with sequence order reversed."""
aligns = pairwise2.align.globalxx("GAT", "GAACT")
self.assertEqual(len(aligns), 2)
aligns.sort()
seq1, seq2, score, begin, end = aligns[0]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
G-A-T
| | |
GAACT
Score=3
""",
)
seq1, seq2, score, begin, end = aligns[1]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
GA--T
|| |
GAACT
Score=3
""",
)
def test_one_alignment_only(self):
"""Test one_alignment_only parameter."""
aligns = pairwise2.align.globalxx("ACCGT", "ACG")
self.assertEqual(len(aligns), 2)
aligns = pairwise2.align.globalxx("ACCGT", "ACG", one_alignment_only=True)
self.assertEqual(len(aligns), 1)
def test_list_input(self):
"""Do a global alignment with sequences supplied as lists."""
aligns = pairwise2.align.globalxx(
["Gly", "Ala", "Thr"], ["Gly", "Ala", "Ala", "Cys", "Thr"], gap_char=["---"]
)
aligns.sort()
seq1, seq2, score, begin, end = aligns[0]
self.assertEqual(score, 3)
self.assertEqual(seq1, ["Gly", "---", "Ala", "---", "Thr"])
self.assertEqual(seq2, ["Gly", "Ala", "Ala", "Cys", "Thr"])
class TestPairwiseLocal(unittest.TestCase):
"""Test some simple local alignments."""
def setUp(self):
self.blosum62 = substitution_matrices.load("BLOSUM62")
def test_localxs_1(self):
"""Test localxx."""
aligns = sorted(pairwise2.align.localxs("AxBx", "zABz", -0.1, 0))
# From Biopython 1.74 on this should only give one alignment, since
# we disallow leading and trailing 'zero-extensions'
self.assertEqual(len(aligns), 1)
seq1, seq2, score, begin, end = aligns[0]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
1 AxB
| |
2 A-B
Score=1.9
""",
)
def test_localxs_2(self):
"""Test localxx with ``full_sequences=True``."""
aligns = sorted(pairwise2.align.localxs("AxBx", "zABz", -0.1, 0))
# From Biopython 1.74 on this should only give one alignment, since
# we disallow leading and trailing 'zero-extensions'
self.assertEqual(len(aligns), 1)
seq1, seq2, score, begin, end = aligns[0]
alignment = pairwise2.format_alignment(
seq1, seq2, score, begin, end, full_sequences=True
)
self.assertEqual(
alignment,
"""\
-AxBx
| |
zA-Bz
Score=1.9
""", # noqa: W291
)
def test_localds_zero_score_segments_symmetric(self):
"""Test if alignment is independent on direction of sequence."""
aligns1 = pairwise2.align.localds(
"CWHISLKM", "CWHGISGLKM", self.blosum62, -11, -1
)
aligns2 = pairwise2.align.localds(
"MKLSIHWC", "MKLGSIGHWC", self.blosum62, -11, -1
)
self.assertEqual(len(aligns1), len(aligns2))
def test_localxs_generic(self):
"""Test the generic method with local alignments."""
aligns = sorted(
pairwise2.align.localxs("AxBx", "zABz", -0.1, 0, force_generic=True)
)
# From Biopython 1.74 on this should only give one alignment, since
# we disallow leading and trailing 'zero-extensions'
self.assertEqual(len(aligns), 1)
seq1, seq2, score, begin, end = aligns[0]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
1 AxB
| |
2 A-B
Score=1.9
""",
)
def test_localms(self):
"""Two different local alignments."""
aligns = sorted(
pairwise2.align.localms("xxxABCDxxx", "zzzABzzCDz", 1, -0.5, -3, -1)
)
alignment = pairwise2.format_alignment(*aligns[0])
self.assertEqual(
alignment,
"""\
6 CD
||
8 CD
Score=2
""",
)
alignment = pairwise2.format_alignment(*aligns[1])
self.assertEqual(
alignment,
"""\
4 AB
||
4 AB
Score=2
""",
)
def test_blosum62(self):
"""Test localds with blosum62."""
self.assertEqual(1, self.blosum62[("K", "Q")])
self.assertEqual(4, self.blosum62[("A", "A")])
self.assertEqual(8, self.blosum62[("H", "H")])
alignments = pairwise2.align.localds(
"VKAHGKKV", "FQAHCAGV", self.blosum62, -4, -4
)
for a in alignments:
self.assertEqual(
pairwise2.format_alignment(*a), "2 KAH\n .||\n2 QAH\n Score=13\n"
)
def test_empty_result(self):
"""Return no alignment."""
self.assertEqual(pairwise2.align.localxx("AT", "GC"), [])
class TestScoreOnly(unittest.TestCase):
"""Test paramater ``score_only``."""
def test_score_only_global(self):
"""Test ``score_only`` in a global alignment."""
aligns1 = pairwise2.align.globalxx("GAACT", "GAT")
aligns2 = pairwise2.align.globalxx("GAACT", "GAT", score_only=True)
self.assertEqual(aligns1[0][2], aligns2)
def test_score_only_local(self):
"""Test ``score_only`` in a local alignment."""
aligns1 = pairwise2.align.localms("xxxABCDxxx", "zzzABzzCDz", 1, -0.5, -3, -1)
aligns2 = pairwise2.align.localms(
"xxxABCDxxx", "zzzABzzCDz", 1, -0.5, -3, -1, score_only=True
)
self.assertEqual(aligns1[0][2], aligns2)
class TestPairwiseOpenPenalty(unittest.TestCase):
"""Alignments with gap-open penalty."""
def test_match_score_open_penalty1(self):
"""Test 1."""
aligns = pairwise2.align.globalms("AA", "A", 2.0, -1, -0.1, 0)
self.assertEqual(len(aligns), 2)
aligns.sort()
seq1, seq2, score, begin, end = aligns[0]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
AA
|
-A
Score=1.9
""",
)
seq1, seq2, score, begin, end = aligns[1]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
AA
|
A-
Score=1.9
""", # noqa: W291
)
def test_match_score_open_penalty2(self):
"""Test 2."""
aligns = pairwise2.align.globalms("GAA", "GA", 1.5, 0, -0.1, 0)
self.assertEqual(len(aligns), 2)
aligns.sort()
seq1, seq2, score, begin, end = aligns[0]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
GAA
| |
G-A
Score=2.9
""",
)
seq1, seq2, score, begin, end = aligns[1]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
GAA
||
GA-
Score=2.9
""", # noqa: W291
)
def test_match_score_open_penalty3(self):
"""Test 3."""
aligns = pairwise2.align.globalxs("GAACT", "GAT", -0.1, 0)
self.assertEqual(len(aligns), 1)
seq1, seq2, score, begin, end = aligns[0]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
GAACT
|| |
GA--T
Score=2.9
""",
)
def test_match_score_open_penalty4(self):
"""Test 4."""
aligns = pairwise2.align.globalms("GCT", "GATA", 1, -2, -0.1, 0)
self.assertEqual(len(aligns), 1)
seq1, seq2, score, begin, end = aligns[0]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
GC-T-
| |
G-ATA
Score=1.7
""", # noqa: W291
)
class TestPairwiseExtendPenalty(unittest.TestCase):
"""Alignments with gap-extend penalties."""
def test_extend_penalty1(self):
"""Test 1."""
aligns = pairwise2.align.globalxs("GACT", "GT", -0.5, -0.2)
self.assertEqual(len(aligns), 1)
seq1, seq2, score, begin, end = aligns[0]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
GACT
| |
G--T
Score=1.3
""",
)
def test_extend_penalty2(self):
"""Test 2."""
aligns = pairwise2.align.globalxs("GACT", "GT", -1.5, -0.2)
self.assertEqual(len(aligns), 1)
aligns.sort()
seq1, seq2, score, begin, end = aligns[0]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
GACT
| |
G--T
Score=0.3
""",
)
class TestPairwisePenalizeExtendWhenOpening(unittest.TestCase):
"""Alignment with ``penalize_extend_when_opening``."""
def test_penalize_extend_when_opening(self):
"""Add gap-extend penalty to gap-opening penalty."""
aligns = pairwise2.align.globalxs(
"GACT", "GT", -0.2, -1.5, penalize_extend_when_opening=1
)
self.assertEqual(len(aligns), 1)
seq1, seq2, score, begin, end = aligns[0]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
GACT
| |
G--T
Score=-1.2
""",
)
class TestPairwisePenalizeEndgaps(unittest.TestCase):
"""Alignments with end-gaps penalized or not."""
def test_penalize_end_gaps(self):
"""Turn off end-gap penalties."""
aligns = pairwise2.align.globalxs("GACT", "GT", -0.8, -0.2, penalize_end_gaps=0)
self.assertEqual(len(aligns), 3)
aligns.sort()
seq1, seq2, score, begin, end = aligns[0]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
GACT
.|
--GT
Score=1
""",
)
seq1, seq2, score, begin, end = aligns[1]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
GACT
| |
G--T
Score=1
""",
)
seq1, seq2, score, begin, end = aligns[2]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
GACT
|.
GT--
Score=1
""", # noqa: W291
)
def test_penalize_end_gaps2(self):
"""Do the same, but use the generic method (with the same result)."""
aligns = pairwise2.align.globalxs(
"GACT", "GT", -0.8, -0.2, penalize_end_gaps=0, force_generic=True
)
self.assertEqual(len(aligns), 3)
aligns.sort()
seq1, seq2, score, begin, end = aligns[0]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
GACT
.|
--GT
Score=1
""",
)
seq1, seq2, score, begin, end = aligns[1]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
GACT
| |
G--T
Score=1
""",
)
seq1, seq2, score, begin, end = aligns[2]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
GACT
|.
GT--
Score=1
""", # noqa: W291
)
def test_separate_penalize_end_gaps(self):
"""Test alignment where end-gaps are differently penalized."""
align = pairwise2.align.globalms(
"AT", "AGG", 1.0, -0.5, -1.75, -0.25, penalize_end_gaps=(True, False)
)
self.assertEqual(align[0], ("A--T", "AGG-", -1.0, 0, 4))
class TestPairwiseSeparateGapPenalties(unittest.TestCase):
"""Alignments with separate gap-open penalties for both sequences."""
def test_separate_gap_penalties1(self):
"""Test 1."""
aligns = pairwise2.align.localxd("GAT", "GTCT", -0.3, 0, -0.8, 0)
self.assertEqual(len(aligns), 2)
aligns.sort()
seq1, seq2, score, begin, end = aligns[0]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
G-AT
| .|
GTCT
Score=1.7
""",
)
seq1, seq2, score, begin, end = aligns[1]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
GA-T
|. |
GTCT
Score=1.7
""",
)
def test_separate_gap_penalties2(self):
"""Test 2."""
aligns = pairwise2.align.localxd("GAT", "GTCT", -0.5, 0, -0.2, 0)
self.assertEqual(len(aligns), 1)
seq1, seq2, score, begin, end = aligns[0]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
1 GAT
| |
1 G-T
Score=1.8
""",
)
class TestPairwiseSeparateGapPenaltiesWithExtension(unittest.TestCase):
"""Alignments with separate gap-extension penalties for both sequences."""
def test_separate_gap_penalties_with_extension(self):
"""Test separate gap-extension penalties and list input."""
aligns = pairwise2.align.localxd(
list("GAAT"), list("GTCCT"), -0.1, 0, -0.1, -0.1, gap_char=["-"]
)
self.assertEqual(len(aligns), 3)
aligns.sort()
seq1, seq2, score, begin, end = aligns[0]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
G - A A T
| . . |
G T C C T
Score=1.9
""", # noqa: W291
)
seq1, seq2, score, begin, end = aligns[1]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
G A - A T
| . . |
G T C C T
Score=1.9
""", # noqa: W291
)
seq1, seq2, score, begin, end = aligns[2]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
G A A - T
| . . |
G T C C T
Score=1.9
""", # noqa: W291
)
class TestPairwiseMatchDictionary(unittest.TestCase):
"""Alignments with match dictionaries."""
match_dict = {("A", "A"): 1.5, ("A", "T"): 0.5, ("T", "T"): 1.0}
def test_match_dictionary1(self):
"""Test 1."""
aligns = pairwise2.align.localds("ATAT", "ATT", self.match_dict, -0.5, 0)
self.assertEqual(len(aligns), 2)
aligns.sort()
seq1, seq2, score, begin, end = aligns[0]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
ATAT
|| |
AT-T
Score=3
""",
)
seq1, seq2, score, begin, end = aligns[1]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
1 ATA
||.
1 ATT
Score=3
""",
)
def test_match_dictionary2(self):
"""Test 2."""
aligns = pairwise2.align.localds("ATAT", "ATT", self.match_dict, -1, 0)
self.assertEqual(len(aligns), 1)
seq1, seq2, score, begin, end = aligns[0]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
1 ATA
||.
1 ATT
Score=3
""",
)
def test_match_dictionary3(self):
"""Test 3."""
aligns = pairwise2.align.localds("ATT", "ATAT", self.match_dict, -1, 0)
self.assertEqual(len(aligns), 1)
seq1, seq2, score, begin, end = aligns[0]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
1 ATT
||.
1 ATA
Score=3
""",
)
class TestPairwiseOneCharacter(unittest.TestCase):
"""Alignments where one sequence has length 1."""
def test_align_one_char1(self):
"""Test sequence with only one match."""
aligns = pairwise2.align.localxs("abcde", "c", -0.3, -0.1)
self.assertEqual(len(aligns), 1)
seq1, seq2, score, begin, end = aligns[0]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
3 c
|
1 c
Score=1
""",
)
def test_align_one_char2(self):
"""Test sequences with two possible match positions."""
aligns = pairwise2.align.localxs("abcce", "c", -0.3, -0.1)
self.assertEqual(len(aligns), 2)
aligns.sort()
seq1, seq2, score, begin, end = aligns[0]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
4 c
|
1 c
Score=1
""",
)
seq1, seq2, score, begin, end = aligns[1]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
3 c
|
1 c
Score=1
""",
)
def test_align_one_char3(self):
"""Like test 1, but global alignment."""
aligns = pairwise2.align.globalxs("abcde", "c", -0.3, -0.1)
self.assertEqual(len(aligns), 1)
seq1, seq2, score, begin, end = aligns[0]
alignment = pairwise2.format_alignment(seq1, seq2, score, begin, end)
self.assertEqual(
alignment,
"""\
abcde
|
--c--
Score=0.2
""", # noqa: W291
)
class TestPersiteGapPenalties(unittest.TestCase):
"""Check gap penalty callbacks use correct gap opening position.
This tests that the gap penalty callbacks are really being used
with the correct gap opening position.
"""
def test_gap_here_only_1(self):
"""Open a gap in second sequence only."""
seq1 = "AAAABBBAAAACCCCCCCCCCCCCCAAAABBBAAAA"
seq2 = "AABBBAAAACCCCAAAABBBAA"
def no_gaps(x, y):
"""Very expensive to open a gap in seq1."""
return -2000 - y
def specific_gaps(x, y):
"""Very expensive to open a gap in seq2.
...unless it is in one of the allowed positions:
"""
breaks = [0, 11, len(seq2)]
return (-2 - y) if x in breaks else (-2000 - y)
alignments = pairwise2.align.globalmc(seq1, seq2, 1, -1, no_gaps, specific_gaps)
self.assertEqual(len(alignments), 1)
formatted = pairwise2.format_alignment(*alignments[0])
self.assertEqual(
formatted,
"""\
AAAABBBAAAACCCCCCCCCCCCCCAAAABBBAAAA
||||||||||| |||||||||||
--AABBBAAAACC----------CCAAAABBBAA--
Score=2
""", # noqa: W291
)
def test_gap_here_only_2(self):
"""Force a bad alignment.
Forces a bad alignment by having a very expensive gap penalty
where one would normally expect a gap, and a cheap gap penalty
in another place.
"""
seq1 = "AAAABBBAAAACCCCCCCCCCCCCCAAAABBBAAAA"
seq2 = "AABBBAAAACCCCAAAABBBAA"
def no_gaps(x, y):
"""Very expensive to open a gap in seq1."""
return -2000 - y
def specific_gaps(x, y):
"""Very expensive to open a gap in seq2.
...unless it is in one of the allowed positions:
"""
breaks = [0, 3, len(seq2)]
return (-2 - y) if x in breaks else (-2000 - y)
alignments = pairwise2.align.globalmc(seq1, seq2, 1, -1, no_gaps, specific_gaps)
self.assertEqual(len(alignments), 1)
formatted = pairwise2.format_alignment(*alignments[0])
self.assertEqual(
formatted,
"""\
AAAABBBAAAACCCCCCCCCCCCCCAAAABBBAAAA
||| ......|||||||||||||
--AAB----------BBAAAACCCCAAAABBBAA--
Score=-10
""", # noqa: W291
)
class TestOtherFunctions(unittest.TestCase):
"""Test remaining non-tested private methods."""
def test_clean_alignments(self):
"""``_clean_alignments`` removes redundant and wrong alignments."""
alns = [
("ACCGT", "AC-G-", 3.0, 0, 4),
("ACCGT", "AC-G-", 3.0, 1, 1),
("ACCGT", "A-CG-", 3.0, 0, 4),
("ACCGT", "AC-G-", 3.0, 0, 4),
("ACCGT", "A-CG-", 3.0, 0, 4),
]
expected = [("ACCGT", "AC-G-", 3.0, 0, 4), ("ACCGT", "A-CG-", 3.0, 0, 4)]
result = pairwise2._clean_alignments(alns)
self.assertEqual(expected, result)
def test_print_matrix(self):
"""``print_matrix`` prints nested lists as nice matrices."""
import sys
from io import StringIO
out = StringIO()
sys.stdout = out
pairwise2.print_matrix(
[
[0.0, -1.0, -1.5, -2.0],
[-1.0, 4.0, 3.0, 2.5],
[-1.5, 3.0, 8.0, 7.0],
[-2.0, 2.5, 7.0, 6.0],
[-2.5, 2.0, 6.5, 11.0],
[-3.0, 1.5, 6.0, 10.0],
]
)
self.assertEqual(
out.getvalue(),
" 0.0 -1.0 -1.5 -2.0 \n"
"-1.0 4.0 3.0 2.5 \n"
"-1.5 3.0 8.0 7.0 \n"
"-2.0 2.5 7.0 6.0 \n"
"-2.5 2.0 6.5 11.0 \n"
"-3.0 1.5 6.0 10.0 \n",
)
sys.stdout = sys.__stdout__
def test_recover_alignments(self):
"""One possible start position in local alignment is not a match."""
self.assertEqual(len(pairwise2.align.localxx("AC", "GA")), 1)
if __name__ == "__main__":
if pairwise2.rint != pairwise2._python_rint:
# This uses the default C extensions, if import didn't fail.
runner = unittest.TextTestRunner(verbosity=2)
unittest.main(testRunner=runner, exit=False)
else:
print(
"Import of C functions failed. Only testing pure Python "
"fallback functions."
)
# Now, we switch explicitly to the fallback Python functions:
pairwise2._make_score_matrix_fast = pairwise2._python_make_score_matrix_fast
pairwise2.rint = pairwise2._python_rint
runner = unittest.TextTestRunner(verbosity=2)
unittest.main(testRunner=runner)