diff --git a/changelog/fix_use_different_symbol_in_ruby_3.md b/changelog/fix_use_different_symbol_in_ruby_3.md
new file mode 100644
index 00000000000..8f0b52c2dda
--- /dev/null
+++ b/changelog/fix_use_different_symbol_in_ruby_3.md
@@ -0,0 +1 @@
+* [#10353](https://github.com/rubocop/rubocop/pull/10353): Use `:ambiguous_regexp` to detect ambiguous Regexp in Ruby 3. ([@danieldiekmeier][], [@joergschiller][])
diff --git a/lib/rubocop/cop/lint/ambiguous_regexp_literal.rb b/lib/rubocop/cop/lint/ambiguous_regexp_literal.rb
index b411791a95b..11985e37091 100644
--- a/lib/rubocop/cop/lint/ambiguous_regexp_literal.rb
+++ b/lib/rubocop/cop/lint/ambiguous_regexp_literal.rb
@@ -30,7 +30,11 @@ class AmbiguousRegexpLiteral < Base
 
         def on_new_investigation
           processed_source.diagnostics.each do |diagnostic|
-            next unless diagnostic.reason == :ambiguous_literal
+            if target_ruby_version >= 3.0
+              next unless diagnostic.reason == :ambiguous_regexp
+            else
+              next unless diagnostic.reason == :ambiguous_literal
+            end
 
             offense_node = find_offense_node_by(diagnostic)
 
diff --git a/spec/rubocop/cop/lint/ambiguous_regexp_literal_spec.rb b/spec/rubocop/cop/lint/ambiguous_regexp_literal_spec.rb
index 1226cf639e9..a11a1c5b003 100644
--- a/spec/rubocop/cop/lint/ambiguous_regexp_literal_spec.rb
+++ b/spec/rubocop/cop/lint/ambiguous_regexp_literal_spec.rb
@@ -1,7 +1,7 @@
 # frozen_string_literal: true
 
 RSpec.describe RuboCop::Cop::Lint::AmbiguousRegexpLiteral, :config do
-  context 'with a regexp literal in the first argument' do
+  shared_examples 'with a regexp literal in the first argument' do
     context 'without parentheses' do
       it 'registers an offense and corrects when single argument' do
         expect_offense(<<~RUBY)
@@ -178,4 +178,12 @@ class MyTest
       end
     end
   end
+
+  context 'Ruby <= 2.7', :ruby27 do
+    include_examples 'with a regexp literal in the first argument'
+  end
+
+  context 'Ruby >= 3.0', :ruby30 do
+    include_examples 'with a regexp literal in the first argument'
+  end
 end