diff --git a/mlflow/projects/utils.py b/mlflow/projects/utils.py
index bfd9f3b2af94f..fc1bfd7943e99 100644
--- a/mlflow/projects/utils.py
+++ b/mlflow/projects/utils.py
@@ -50,16 +50,21 @@
 def _parse_subdirectory(uri):
     # Parses a uri and returns the uri and subdirectory as separate values.
     # Uses '#' as a delimiter.
+    unquoted_uri = _strip_quotes(uri)
     subdirectory = ""
-    parsed_uri = uri
-    if "#" in uri:
-        subdirectory = uri[uri.find("#") + 1 :]
-        parsed_uri = uri[: uri.find("#")]
+    parsed_uri = unquoted_uri
+    if "#" in unquoted_uri:
+        subdirectory = unquoted_uri[unquoted_uri.find("#") + 1 :]
+        parsed_uri = unquoted_uri[: unquoted_uri.find("#")]
     if subdirectory and "." in subdirectory:
         raise ExecutionException("'.' is not allowed in project subdirectory paths.")
     return parsed_uri, subdirectory
 
 
+def _strip_quotes(uri):
+    return uri.strip("'\"")
+
+
 def _get_storage_dir(storage_dir):
     if storage_dir is not None and not os.path.exists(storage_dir):
         os.makedirs(storage_dir)
diff --git a/tests/projects/test_utils.py b/tests/projects/test_utils.py
index 1272cb9a79a55..f6d41e7b5fc67 100644
--- a/tests/projects/test_utils.py
+++ b/tests/projects/test_utils.py
@@ -137,6 +137,12 @@ def test_parse_subdirectory():
     assert parsed_uri == "uri"
     assert parsed_subdirectory == "subdirectory"
 
+    # Make sure the parsing works with quotes.
+    test_uri = "'uri#subdirectory'"
+    parsed_uri, parsed_subdirectory = _parse_subdirectory(test_uri)
+    assert parsed_uri == "uri"
+    assert parsed_subdirectory == "subdirectory"
+
     # Make sure periods are restricted in Git repo subdirectory paths.
     period_fail_uri = GIT_PROJECT_URI + "#.."
     with pytest.raises(ExecutionException):