diff --git a/src/Illuminate/Testing/AssertableJsonString.php b/src/Illuminate/Testing/AssertableJsonString.php
index 8819315ec7a0..92962fa55d5d 100644
--- a/src/Illuminate/Testing/AssertableJsonString.php
+++ b/src/Illuminate/Testing/AssertableJsonString.php
@@ -3,6 +3,7 @@
 namespace Illuminate\Testing;
 
 use ArrayAccess;
+use Closure;
 use Countable;
 use Illuminate\Contracts\Support\Jsonable;
 use Illuminate\Support\Arr;
@@ -219,7 +220,11 @@ public function assertMissingExact(array $data)
      */
     public function assertPath($path, $expect)
     {
-        PHPUnit::assertSame($expect, $this->json($path));
+        if ($expect instanceof Closure) {
+            PHPUnit::assertTrue($expect($this->json($path)));
+        } else {
+            PHPUnit::assertSame($expect, $this->json($path));
+        }
 
         return $this;
     }
diff --git a/tests/Testing/TestResponseTest.php b/tests/Testing/TestResponseTest.php
index e054a875a68e..887ba4a0516b 100644
--- a/tests/Testing/TestResponseTest.php
+++ b/tests/Testing/TestResponseTest.php
@@ -827,6 +827,27 @@ public function testAssertJsonPathCanFail()
         $response->assertJsonPath('0.id', '10');
     }
 
+    public function testAssertJsonPathWithClosure()
+    {
+        $response = TestResponse::fromBaseResponse(new Response([
+            'data' => ['foo' => 'bar'],
+        ]));
+
+        $response->assertJsonPath('data.foo', fn ($value) => $value === 'bar');
+    }
+
+    public function testAssertJsonPathWithClosureCanFail()
+    {
+        $response = TestResponse::fromBaseResponse(new Response([
+            'data' => ['foo' => 'bar'],
+        ]));
+
+        $this->expectException(AssertionFailedError::class);
+        $this->expectExceptionMessage('Failed asserting that false is true.');
+
+        $response->assertJsonPath('data.foo', fn ($value) => $value === null);
+    }
+
     public function testAssertJsonFragment()
     {
         $response = TestResponse::fromBaseResponse(new Response(new JsonSerializableSingleResourceStub));