diff --git a/starlette/datastructures.py b/starlette/datastructures.py
index 2c5c4b016..fb715e656 100644
--- a/starlette/datastructures.py
+++ b/starlette/datastructures.py
@@ -421,17 +421,13 @@ class UploadFile:
     def __init__(
         self,
         filename: str,
-        file: typing.Optional[typing.BinaryIO] = None,
         content_type: str = "",
         *,
         headers: "typing.Optional[Headers]" = None,
     ) -> None:
         self.filename = filename
         self.content_type = content_type
-        if file is None:
-            self.file = tempfile.SpooledTemporaryFile(max_size=self.spool_max_size)  # type: ignore  # noqa: E501
-        else:
-            self.file = file
+        self.file = tempfile.SpooledTemporaryFile(max_size=self.spool_max_size)  # type: ignore  # noqa: E501
         self.headers = headers or Headers()
 
     @property
diff --git a/tests/test_datastructures.py b/tests/test_datastructures.py
index b110aa8bd..38d67dc47 100644
--- a/tests/test_datastructures.py
+++ b/tests/test_datastructures.py
@@ -1,5 +1,3 @@
-import io
-
 import pytest
 
 from starlette.datastructures import (
@@ -227,21 +225,9 @@ async def test_upload_file():
     await big_file.close()
 
 
-@pytest.mark.anyio
-async def test_upload_file_file_input():
-    """Test passing file/stream into the UploadFile constructor"""
-    stream = io.BytesIO(b"data")
-    file = UploadFile(filename="file", file=stream)
-    assert await file.read() == b"data"
-    await file.write(b" and more data!")
-    assert await file.read() == b""
-    await file.seek(0)
-    assert await file.read() == b"data and more data!"
-
-
 def test_formdata():
-    stream = io.BytesIO(b"data")
-    upload = UploadFile(filename="file", file=stream)
+    upload = UploadFile(filename="file")
+    upload.file.write(b"data")
     form = FormData([("a", "123"), ("a", "456"), ("b", upload)])
     assert "a" in form
     assert "A" not in form